Abstract
Keywords
Introduction
- Gentile P.
- Scioli M.G.
- Bielli A.
- Orlandi A.
- Cervelli A.
Material and methods
Ethics statement
PRP sample collection and preparation

Cytokines quantification
Statistical analysis
Results
Comparative analysis of cytokine and growth factor concentrations in PRP fractions obtained by human thrombin or mechanical activation

The effect of prolonged storage of mechanically-activated PRP without preservation agents on the concentration of anti-inflammatory cytokines and growth factors

Discussion
Author contributions
Funding
Ethics approval
Declaration of Competing Interest
Appendix. Supplementary materials
References
- Treatment with platelet-rich plasma is more effective than placebo for knee osteoarthritis: a prospective, double-blind, randomized trial.Am J Sports Med. 2013; 41: 356-364
- Platelet-rich plasma: why intra-articular? A systematic review of preclinical studies and clinical evidence on PRP for joint degeneration.Knee Surg Sports Traumatol Arthrosc. 2015; 23: 2459-2474
- The use of platelet-rich plasma in the nonsurgical management of sports injuries: hype or hope?.Hematology Am Soc Hematol Educ Program. 2013; 2013: 620-626
- The role of cytokines in osteoarthritis pathophysiology.Biorheology. 2002; 39: 237-246
- Platelet quantification and growth factor analysis from platelet-rich plasma: implications for wound healing.Plast Reconstr Surg. 2004; 114: 1502-1508
- Transforming growth factor β: biochemistry and roles in embryogenesis, tissue repair and remodeling, and carcinogenesis.Rec Prog Horm Res. 1988; 44: 157-197
- Platelet-rich plasma application in chondrogenesis.Adv Biomed Res. 2014; 3: 138
- A new technique for hemodilution, preparation of autologous platelet-rich plasma and intraoperative blood salvage in cardiac surgery.Int J Artif Organs. 1987; 10: 47-50
- Reconstruction of alar nasal cartilage defects using a tissue engineering technique based on a combined use of autologous chondrocyte micrografts and platelet-rich plasma: preliminary clinical and instrumental evaluation.Plast Reconstr Surg Glob Open. 2016; 4: e1027
- Platelet-rich plasma as treatment for persistent ocular epithelial defects.Transfus Apher Sci. 2015; 52: 300-304
- Autologous platelets as a source of proteins for healing and tissue regeneration.Thromb Haemost. 2004; 91: 4-15
- Classification of platelet concentrates: from pure platelet-rich plasma (P-PRP) to leucocyte- and platelet-rich fibrin (L-PRF).Trends Biotechnol. 2009; 27: 158-167
- Platelet-rich-plasma: preparation and formulation.Oper Tech Orthop. 2012; 22: 25-32
- Actions of thrombin and other coagulant and proteolytic enzymes on blood platelets.Nature. 1967; 216: 857-858
- Platelet-rich plasma: the choice of activation method affects the release of bioactive molecules.Biomed Res Int. 2016; 20166591717
- Generation of autologous platelet-rich plasma by the ultrasonic standing waves.IEEE Trans Biomed Eng. 2016; 63: 1642-1652
- A cycle of freezing and thawing as a modified method for activating platelets in platelet-rich plasma to use in regenerative medicine.Panam J Trauma, Crit Care Emer Surg. 2020; 9: 101-104
- The influence of time of storage, temperature of storage, platelet number in platelet-rich plasma, packed cell, mean platelet volume, hemoglobin concentration, age, and sex on platelet aggregation test.Ann Hematol. 1995; 71: 129-133
- The effect of four different freezing conditions and time in frozen storage on the concentration of commonly measured growth factors and enzymes in equine platelet-rich plasma over six months.BMC Vet Res. 2019; 15: 292
- Does platelet-rich plasma freeze-thawing influence growth factor release and their effects on chondrocytes and synoviocytes?.Biomed Res Int. 2014; 2014692913
- Insulin-like growth factor-1 endues monocytes with immune suppressive ability to inhibit inflammation in the intestine.Sci Rep. 2015; 5: 7735
- TGF-Β1-β: regulation of extracellular matrix.Kidney Int. 1992; 41: 557-559
- Basic science and clinical application of platelet-rich plasma for cartilage defects and osteoarthritis: a review.Osteoarthritis Cartilage. 2013; 21: 1627-1637
- Platelet-rich plasma: from basic science to clinical applications.Am J Sports Med. 2009; 37: 2259-2272
- An evaluation of the effect of activation methods on the release of growth factors from platelet-rich plasma.Plast Reconstr Surg. 2022; 149: 404-411
- Evaluation of not-activated and activated PRP in hair loss treatment: role of growth factor and cytokine concentrations obtained by different collection systems.Int J Mol Sci. 2017; 18: 408
- Freeze-dried human platelet-rich plasma retains activation and growth factor expression after an eight-week preservation period.Asian Spine J. 2017; 11: 329-336
- Functional assessment of autologous platelet-rich plasma (PRP) after long-term storage at −20 °C without any preservation agent.J Plast Surg Hand Surg. 2017; 51: 235-239
- Activation of equine platelet-rich plasma: comparison of methods and characterization of equine autologous thrombin.Vet Surg. 2012; 41: 784-794
- Inflammatory cytokine concentrations are acutely increased by hyperglycemia in humans: role of oxidative stress.Circulation. 2002; 106: 2067-2072
- Relationships of age and sex with cytokine content and distribution in human platelet fibrin gels.Sci Rep. 2018; 8: 10642
- Effects of solution conditions, processing parameters, and container materials on aggregation of a monoclonal antibody during freeze-thawing.J Pharm Sci. 2008; 97: 1801-1812
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